early and late effects of ibuprofen on mouse sperm parameters, chromatin condensation, and dna integrity in mice
نویسندگان
چکیده
background: there are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and dna integrity. objective: to determine the effects of ibuprofen on sperm parameters, chromatin condensation and dna integrity of mice. materials and methods: in this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control (group i, n=12), normal dosage of ibuprofen (group ii, n=12) and high dosage (group iii, n=12). ibuprofen with different doses was dissolved in daily water of animals. after 35, 70 and 105 days, the cauda epididymis of mice were cut and incubated in ham’s f10 media. sperm samples were analyzed for parameters (motility, morphology and count), dna integrity (scd test) and chromatin condensation (chromomycin a3 and aniline blue staining). results: after 35 days, in addition to above mentioned sperm parameters, all of the treated mice showed statistically significant increase in spermatozoa with immature chromatin (p<0.05). however, after 70 days, the rate of sperm dna fragmentation assessed by scd was increased in group ii (66.5±0.7) and the percentage of immature spermatozoa (ab+ and cma3+) was higher in group iii (77.5±0.7 and 49.5±6.3 respectively) than other groups. after 105 days, the ab+ spermatozoa were increased in both normal dose and high dose groups. conclusion: ibuprofen may cause a significant reduction in sperm parameters and sperm chromatin/dna integrity in mice. it should be noted that these deleterious effects are dose-dependent and can be seen in early and late stage of drug treatments.
منابع مشابه
Early and late effects of Ibuprofen on mouse sperm parameters, chromatin condensation, and DNA integrity in mice.
BACKGROUND There are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and DNA integrity. OBJECTIVE To determine the effects of Ibuprofen on sperm parameters, chromatin condensation and DNA integrity of mice. MATERIALS AND METHODS In this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control ...
متن کاملP-1: Morphine Has Detrimental Effects on Sperm Parameters, Chromatin Quality and DNA Integrity in Mice
Background Morphine as a natural alkaloid (opiate) is considered as one of the problems associated with poor semen production and sperm quality. For the first time, this experimental study was carried out to evaluate the impact of intra-peritoneal injection of morphine on sperm parameters, chromatin quality and DNA integrity of spermatozoa aspirated from cauda epididymis of mice. MaterialsAndMe...
متن کاملEffect of chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men
Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic material of sperm is beneficial for determination of the fertility of sperm. The aim of this study was to assess the effect of sperm chromatin co...
متن کاملP-18: Effects of Ethanol Consumption on Chromatin Condensation and DNA Integrity of Epididymal Spermatozoa In Rat
Background: Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Both acute and chronic alcohol consumption may affect spermatozoal chromatin disorders through apoptosis. Therefore, for the first time, this experimental study was performed to evaluate the effect of ethanol consumption on sperm parameters and chromatin integrity of spermatoz...
متن کاملthe effect of varicocelectomy on sperm parameters, membrane integrity and sperm chromatin condensation
introduction: varicocele is considered as a common cause of male infertility. the aim of this study was to evaluate the effects of varicocelectomy on sperm parameters, sperm membrane integrity and sperm nuclear maturity. materials and methods: in this study along with routine semen analysis, hypo-osmotic swelling test (host), and aniline blue staining for assessment of as 45 and 90 days after i...
متن کاملP-61: Effects of Acrylamid on Sperm Parameters and Chromatin Quality in Mice
Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm paramete...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
international journal of reproductive biomedicineجلد ۱۳، شماره ۱۱، صفحات ۷۰۳-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023